Pubdate: Fri, 31 Aug 2001 Source: Journal of the American Medical Association (US) Copyright: 2001 American Medical Association. Contact: http://jama.ama-assn.org/ Details: http://www.mapinc.org/media/219 Author: Brian Vastag PAY ATTENTION: RITALIN ACTS MUCH LIKE COCAINE WashingtonAdvanced imaging research has answered a 40-year-old question about methylphenidate (Ritalin), which is taken daily by 4 million to 6 million children in the United States: how does it work? The answer may unsettle many parents, because the drug acts much like cocaine, albeit cocaine dripped through molasses (J Neurosci. 2001;21:RC121). Taken orally in pill form, methylphenidate rarely produces a high and has not been reported to be addictive. However, injected as a liquid it sends a jolt that "addicts like very much," said Nora Volkow, MD, psychiatrist and imaging expert at Brookhaven National Laboratory, Upton, NY. "They say it's like cocaine." Representative distribution volume PET images of the radiotracer [11C]raclopride from one of the study participants show that radiotracer binding is reduced at the level of the striatum (bottom left) after oral administration of 60 mg of methylphenidate. Reduced radiotracer binding indicates decreased availability of open dopamine receptors after methylphenidate-induced increases in extracellular dopamine. Cocaine produces a similar effect in those who take it. (Photo credit: Courtesy of Brookhaven National Laboratory) Acknowledged as leaders in the field of brain imaging of drug effects, Volkow and colleagues have spent several years tracing the effects on the brain of drugs of addiction, using positron emission tomography (PET) and other advanced techniques. Among their long list of findings, they've identified the brain's dopamine system as a major player in compulsive behavior, including drug taking and overeating. A PRAGMATIC PARADOX Building on that base, Volkow, associate laboratory director for life sciences at Brookhaven, hit the trail of a legal stimulant. Although they have used it to treat attention-deficit/hyperactivity disorder (ADHD) for 40 years, psychiatrists and pharmacologists have never known how or why it worked. Chemically similar to cocaine and other stimulants, methylphenidate presents a pragmatic paradox: it decreases activity and increases the ability to concentrate in people with ADHD, but in studies, about half of those without ADHD find it unpleasant, like drinking too much coffee. "I've almost been obsessed about trying to understand [methylphenidate] with imaging," said Volkow at a recent media conference. "As a psychiatrist, sometimes I feel embarrassed [about the lack of knowledge] because this is, by far, the drug we prescribe most frequently to children." So the team went to work with PET scans to examine the dopamine system, which stimulates reward and motivation circuits during pleasurable experienceseating, having sex, learning. To pick one of many pleasures, tasting chocolate ice cream will trigger cells in the basal ganglia to release dopamine molecules. These float across the synapse to neurons in a reward circuit. Receptors on these cells sop up the dopamine, activating signals that translate to "this experience is worth paying attention to." Too much signal and the experience feels unpleasant, overstimulating. Too little, and the experience elicits a yawn; no pleasure, only boredom and distraction. Volkow wanted to know how methylphenidate affects this signal. But instead of focusing on dopamine receptors, she tracked another part of the system. After the pleasure signal is sent on its way, dopamine molecules recycle back to the neurons that produced them. There, transportersalso called autoreceptorsact as vacuum cleaners, scouring the synapse for another go-around. Earlier research had shown that cocaine blocks about 50% of these transporters, leading to a surfeit of dopamine in the synapse and a hit of pleasure. Because of methylphenidate's chemical similarities to cocaine, pharmacologists thought that it might work in the same way, only less potently, blocking fewer transporters. Animal studies with high doses of methylphenidate indicated that this could be the case. STARTLING RESULTS Using a radiotracer, [11C]raclopride, that labels dopamine transporters, the team scanned 11 healthy men who took various doses of oral methylphenidate. The results were shocking. "We were surprised as hell," said Volkow. "We didn't expect this." Instead of being a less potent transport inhibitor than cocaine, methylphenidate was more potent. A typical dose given to children, 0.5 mg/kg, blocked 70% of dopamine transporters. "The data clearly show that the notion that Ritalin is a weak stimulant is completely incorrect," Volkow said. More pondering led the team to consider two theories. Methylphenidate could be blocking the recycling of dopamine exactly as cocaine does, leading to strong signals that would yield a high and lead to addiction. But this did not jibe with four decades of clinical experience. So they considered another possibility. Perhaps methylphenidate seeps into the brain slowly, and as one by one the drug molecules block the transporters, dopamine cells shift gears. Like a union foreman yelling to an assembly line to slow down, the cell interprets the transporter congestion as a signal that too much dopamine is being produced. The neuron cranks down production, sending less dopamine into the synapse, suppressing the reward signal. The two theories opposed each other. But Volkow was unfazed. "We had to let the data speak for itself," she said. That meant measuring the amount of dopamine floating in the synapses. Fortunately, the investigators had at hand another radioactive label that binds only to open dopamine receptors. A weak PET signal would mean low numbers of open receptors, which in turn would mean that large amounts of dopamine occupied the synapse. After combining data from the volunteers, the team got its second surprise. Those who took methylphenidate displayed high levels of extracellular dopaminejust like people using cocaine. But if methylphenidate works like cocaine, why aren't millions of US children getting high and becoming addicted? CAPTURING THE ANSWER The answer came after Volkow combined her results with those from another research team. In 1999, Darin Dougherty, MD, and colleagues at Massachusetts General Hospital and Harvard University Medical School reported that people with ADHD have many more dopamine transporters than those without the condition (Lancet. 1999;354:2132-2133). This surplus increases the collective cleaning power of each cell; as dopamine fires into the synapse it is quickly sucked back, before it can home in on reward circuit receptors. "There isn't enough time for it to produce a signal," said Volkow. It finally started to make sense. Children with ADHD produce weak dopamine signals, meaning that usually interesting activities provide fewer rewards. In effect, their attention circuitry is underfed. At the same time, they experience a related effect: random, distracting neuron firing. Or, as Volkow put it, more noise and less signal. This background hum interferes with concentration, making the child more distractible. Methylphenidate flips the relationship, upping the signal and reducing the noise. After someone swallows methylphenidate, it enters the bloodstream and eventually finds the brain, where it blocks dopamine transporters and increases attention signaling. Again, cocaine acts the same way. But the two drugs differ in a significant way: methylphenidate takes about an hour to raise dopamine levels, whereas inhaled or injected cocaine hits the brain in seconds. "It is the speed at which you increase dopamine that appears to be a key element of the addiction process," said Volkow. While the team is unclear on why this speed factor is so important, future research will focus on it. They also plan to map dopamine levels in volunteers who have ADHD when they are at rest or while concentrating. Other research will search for molecular tools to screen children for dopamine transporter levels; those with high levels could be identified early and encouraged with behavioral solutions before methylphenidate is prescribed. "We know that social interactions can increase dopamine receptors," said Volkow, but whether better interplay also affects transporter levels is unknown. The long-term dopamine effects of taking methylphenidate for years, as many do, are another unknown. The only two large epidemiological studies conflict. One reports more drug addiction in children with ADHD who took methylphenidate compared with children with ADHD who took no drug (J Learn Disabil. 1998;31:533-544); the other shows the opposite result (Pediatrics. 1999;104:e20). Because people with low levels of dopamine receptors are at risk for drug addiction, Volkow said that researchers need to understand if methylphenidate can alter the whole dynamic of the dopamine pathway. "Could chronic use of Ritalin make you more vulnerable to decreased dopamine brain activity as cocaine does? It's a key question nobody has answered." - --- MAP posted-by: Keith Brilhart